时间：2025年5月28日（周三）上午10:00-11:00

Time：10:00-11:00, Wednesday, May 28, 2025

主持人: 西湖大学交叉科学中心副主任，汤雷翰讲席教授

Host: Prof. Leihan Tang, Chair Professor & Deputy Director of the Center for Interdisciplinary Studies (CIS), Westlake University

地址：西湖大学云谷校区E10-306

Venue: E10-306, Yungu Campus, Westlake University

讲座语言：英文

Lecture Language: English

Prof. Yihan Wan (万奕含)

Principle Investigator of Single molecule dynamic gene expression laboratory
School of Life Sciences, Westlake University

主讲人/Speaker：

Dr. Yihan Wan earned her Ph.D. from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, with a visiting scholar training at Carnegie Institution for Science, Department of Embryology. She then completed postdoctoral research in Dr. Daniel Larson’s lab at the National Cancer Institute, National Institutes of Health, where she developed a high-throughput, single-molecule imaging system for recording nascent RNA dynamics in living cells. Dr. Wan joined Westlake University in May 2022, and her lab's primary research goal is to elucidate the spatiotemporal dynamics of nascent RNA transcription in human cells, starting from the mechanistic behaviour of individual macromolecules and proceeding to their regulation in cells and tissues.

讲座摘要/Abstract:

Real-time imaging of nascent RNA dynamics in live cells reveals transcription bursting behaviour, essential for understanding the spatiotemporal patterns and mechanisms of genetic information flow. However, systematically elucidating dynamic gene expression activity remains challenging. Here, we combine single-molecule nascent RNA imaging, gene barcoding, in situ sequencing and computer vision to capture the real-time transcription dynamics for hundreds of genes in tens of thousands of single cells, enabling a comprehensive analysis of nascent RNA kinetics. Our systematic approach uncovers diverse transcriptional dynamics patterns and dissects the regulatory mechanisms involved, encompassing cis-regulatory elements, epigenetic modifications, chromatin structure, and gene neighbourhoods that shape the temporal patterns of gene expression. Remarkably, we observed shifts in transcriptional dynamic patterns upon neural differentiation of human embryonic stem cells. We further identify and validate key regulatory elements that influence transcription dynamics in this context, demonstrating that altered dynamic patterns— even without changing average expression levels—contribute to neurodevelopmental defects.

讲座联系人/Contact:

交叉科学中心，朱子霖，邮箱：zhuzilin@westlake.edu.cn

Center for Interdisciplinary Studies (CIS), Ms. Zilin Zhu, Email: zhuzilin@westlake.edu.cn